Comparative analysis of long noncoding RNAs in angiosperms and characterization of long noncoding RNAs in response to heat stress in Chinese cabbage

Abstract Long noncoding RNAs (lncRNAs) are widely present in different species and play critical roles response to abiotic stresses. However, the functions of lncRNAs Chinese cabbage under heat stress remain unknown. Here, we first conducted a global comparative analysis 247,242 among 37 species. The results indicated that were poorly conserved species, only 960 homologous 524 miRNA precursors. We then carried out lncRNA sequencing for genome-wide their target genes at stages treatment. In total, 18,253 identified, which 1229 differentially expressed (DE) characterized as being heat-responsive. ceRNA network revealed 38 lncRNAs, 16 miRNAs, 167 mRNAs involved cabbage. Combined cis - trans -regulated targets DE significantly enriched “protein processing endoplasmic reticulum” “plant hormone signal transduction” pathways. Furthermore, majority HSP PYL these two pathways exhibited similar expression patterns responded rapidly. Based on networks lncRNA-mRNAs, 29 22 found interact with genes, respectively. Finally, several was verified by qRT-PCR. Overall, performed comprehensive Our findings expand knowledge cabbage, identified provide an abundance resources future functional studies.